Bacteriophage T4 Initiates Bidirectional DNA Replication through a Two-Step Process
نویسندگان
چکیده
منابع مشابه
DNA helicase requirements for DNA replication during bacteriophage T4 infection.
The lytic bacteriophage T4 uses multiple mechanisms to initiate the replication of its DNA. Initiation occurs predominantly at replication origins at early times of infection, but there is a switch to genetic recombination-dependent initiation at late times of infection. The T4 insertion-substitution system was used to create a deletion in the T4 dda gene, which encodes a 5'-3' DNA helicase tha...
متن کاملCoordinated DNA Replication by the Bacteriophage T4 Replisome
The T4 bacteriophage encodes eight proteins, which are sufficient to carry out coordinated leading and lagging strand DNA synthesis. These purified proteins have been used to reconstitute DNA synthesis in vitro and are a well-characterized model system. Recent work on the T4 replisome has yielded more detailed insight into the dynamics and coordination of proteins at the replication fork. Since...
متن کاملIdentification of two new bacteriophage T4 genes that may have roles in transcription and DNA replication.
We have identified two bacteriophage T4 genes, 45.1 and 45.2, that map in the intergenic space between phage replication genes 46 (which encodes a recombination initiation protein) and 45 (which encodes a bifunctional protein required in replication and transcription). The existence of genes 45.1 and 45.2 had not been previously recognized by mutation analysis of the T4 genome. We cloned the T4...
متن کاملThe carboxy-terminal domain initiates trimerization of bacteriophage T4 fibritin.
Bacteriophage T4 fibritin is a triple-stranded, parallel, segmented alpha-helical coiled-coil protein. Earlier we showed that the C-terminal globular domain (foldon) of fibritin is essential for correct trimerization and folding of the protein. We constructed the chimerical fusion protein W31 in which the fibritin foldon sequence is followed by the small globular non-alpha-helical protein gp31 ...
متن کاملRescue of DNA replication and bacteriophage production after infection with T4 DNA ligase mutants.
By preventing phage DNA synthesis during a critical period, conditions have been found under which DNA replication and phage production are rescued after infection with T4 DNA ligase mutants.
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ژورنال
عنوان ژورنال: Molecular Cell
سال: 1998
ISSN: 1097-2765
DOI: 10.1016/s1097-2765(00)80167-7